Abstract: Objective: By investigating the availability of emergency drugs in medical institutions, it provides a basis for improving the policy of emergency drug supply guarantee in response to public health emergencies. Methods and results: based on the World Health Organization (WHO) and the International Health Initiative (HAI) standard investigation methodology, evaluating the accessibility from 2013 to 2018 of 52 emergency drugs in different medical institutions in Nanjing city. The overall availability of emergency drugs was more than 50%, and the overall trend was on the rise, there were statistical differences in the availability of different level medical institutions (P＜0.001), and the availability in primary medical institutions was the lowest, among 25% to 40%. The Laspeyres price index for emergency drugs had been rising year by year, with the price of drugs in 2018 was 4.8 times than 2013. The affordability of emergency drugs was good, and that of urban residents was better than of rural residents. Conclusion: It is suggested that we should strengthen the emergency drug reserve system and giving full play to the role of primary medical institutions, improving the drug pricing mechanism, and formulate a national list of emergency drugs.