Currently, general hospitals as major diagnosis and treatment units are very important for prevention and control of Novel Coronavirus pneumonia (NCP) in the context of global pandemic of NCP. In this study we would like to discuss about the experiences and deficiencies of the comprehensive strategy and hospital management of NCP prevention and control combining with its own regional characters in designed by The Affiliated Jiangning Hospital of Nanjing Medical University. In addition to provide references for improving strategies of NCP prevention and control.