In order to achieve the epidemic prevention goal of “dynamic zero-COVID”, conducting nucleic acid tests for all citizens is one of the most important measure. To summarize the practical experience of COVID tests in non-government ICL from one metropolis, data and information had been analyzed via field visits and questionnaires. The results showed that 21 non-government ICL participated in the large-scale detection of COVID-19. The maximum daily detection capacity of the 21 laboratories could be expanded from 271,000 to 840,000. A total of 4.227 million samples were tested in 19 days. The main problems found in the survey: the uneven distribution of laboratories in various regions, the incomplete matching of sample distribution and capacity of laboratories in the early stage of the epidemic, the shortage of professional inspection personnel, the untimely clearance of medical waste in some laboratories. It is suggested that the introduction and cultivation of ICL should be attached importance by local governments, test samples should be rationally allocated, the mechanism of inspector reserving should be established, and the ability to predict and dispatch medical waste should be strengthened.