Abstract：In order to understand the meaning of life of medical students and its influencing factors, 671 college students in a medical college in Jiangsu Province were selected to conduct an online survey to provide reference for formulating targeted intervention measures. The results showed that the total score of medical students' sense of meaning of life (101.04±18.65) was in the middle level. The total score of psychological capital and all dimensions were positively correlated with the total score of sense of meaning of life and all dimensions. Regression analysis showed that gender, whether to serve as a student leader, family rearing style and psychological capital were the influencing factors of medical students' sense of meaning of life. The level of medical students' sense of meaning of life needs to be improved. College educators can formulate corresponding educational strategies based on different influencing factors.