The outflow of nurses is becoming more and more serious in recent years. The article firstly analyzed the basic situation and characteristics about the nurses’ outflow from 2006 to 2011 of a top tertiary hospital in Nanjing city by using the method of statistic analysis and telephone follow-up, then discussed the main reasons for the loss, at last proposed some suggestions to solve the problem such as proceeding layer-management and performance management, meeting young nurses’ psychological needs, providing more opportunities of learning and advancement, and improving nurses’ professional status.