Objective:To explore the stability of undergraduate students’ personality deviation across time and its relationship with personality traits. Methods: The basic information scale, personality diagnostic questionnaire-4 and Chinese big five personality inventory were used twice a year among sophomores in the four universities. At last 179 students were been tracked. Results: Repeated analysis of variance indicated that among all the factors, only the antisocial personality deviation was statistically significant(F= 7.055,P < 0.01)across time, the rest dimensions of personality deviation showed no statistically significant. Schzotypal, antisocial, borderline and narcissistic personality deviations were statistically significant of gender main effect (F = 7.682,16.641,7.060,21.420,respectively;P < 0.01, all). Regression analysis suggested that neuroticism trait was significantly positively correlated with personality deviation (t = 6.092, P < 0.01), and agreeableness was significantly negatively correlated with it (t = -2.169, P < 0.05). Conclusion: The dimensions of personality deviation were stable among the university students. Neuroticism and agreeableness traits related to personality deviation.