Objective:To evaluation of clinical pathway applied to 13 diseases in a certain hospital. Methods: Selected the implementation of clinical pathway from 2012 March to 2014 February and unimplementation from 2009 October to 2011 September of bladder tumor in 13 cases as the experimental group and control group, respectively. Set the preoperative hospital days, days of hospital stays, hospitalization expenses and medicine expenses as the assessment indexes by using Pearson correlation analysis and Hotelling T2 or t test. Results:Evaluation indexes of most diseases had a significant positive correlation. On this basis, the difference test also showed that most diseases after the implementation of clinical pathway showed significant effect(P<0.05). Conclusion:The clinical pathway which had a scientific and reasonable standard and strict implementation could effectively reduce the hospitalization days, which improves medical efficiency, and reduces the cost of hospitalization of patients and the medical cost.