Objective:To investigate the college students’ knowledge,attitudes and practices concerning antibiotics use in Nanjing and to provide the evidence for formulating policy on rational use of drugs by analyzing the influencing factors . Methods:A self-designed 5-score Likert scale questionnaire was used to investigate a cluster sampling of 618 students of 5 universities. Results:A total of 600 subjects completed the survey instrument,the correct rate of medication knowledge was 55.07%.Logistic regression analysis identified major,grade and home town as independent risk factors for irrational use concerning antibiotics (P < 0.01). Conclusion: Undergraduate students had inadequate knowledge,moderately accurate beliefs and inappropriate practices concerning antibiotics. Education on medication knowledge for college students especially for non-medical students should be strengthened,so as to change the wrong medication concept and bad habits and improve the rationality of medication practices.