Objective: To investigate on the condition of the nurses’ patience to provide advice to improve nurses’ patience and humanistic caring ability. Methods: The nurses and patients were asked to do survey of the nurses’ patience by the questionnaire we designed. Results: The investigation on nurses and patients showed that nurses made a high evaluation on their patience while the patient made a much lower evaluation on it. The level of nurses’ patience varied in different medical departments and the emergency department was the lowest one (P < 0.05). Above all,working pressure was the main obstacle of the patience. Patients paid the most attention to the manners of nurses. Conclusion: Nurses should strengthen their congnition of humanistic connotation and humanistic caring ability so that they can communicate with patients in a patient way. Hospital managers should learn to allocate the resources in a reasonable way to reduce pressure for nurses.