Objective: Taking the three general hospitals in Jiangsu Province as an example, this paper aimed to analyze the expression condition of medical professional attitude, and provide the basis for doctors to improve the professional manner, and put forward feasible suggestions. Methods: Self-designed questionnaire was performed to survey both doctors and patients from 8 class Ⅲ comprehensive hospitals in Jiangsu Province, and the survey data was analyzed by descriptive analysis and chi-square test. Results: 65.3% of the doctors had been exposed the micro expressions which were not accepted by the patients. The patients showed the highest satisfaction on the doctors’ demeanor of kind and focus. The main factors influencing the doctors’demeanor was work stress. Conclusion: Doctors are lack of the cognition of medical professional attitudes of expression and comprehensive perception; Doctors need to improve the cognition to the expression, strengthen the control of expression, improve the effectiveness of doctor-patient communication, promote the harmonious doctor-patient relationship. Meanwhile, doctors should reduce working pressure, enhance work enthusiasm, improve the professional manner.