Objective: To understand the present situation of scientific research of medical staff in hospitals, and to make reasonable suggestions for improving the research level of modern hospitals. Methods: A questionnaire survey of 222 medical workers in 10 tertiary hospitals in Nanjing was carried out by using the self-designed questionnaire, and the results were analyzed. Results: The ability of scientific research and medical staff complemented each other. The research motivation was closely related to the satisfaction of medical staff. Lack of adequate scientific research time was the main factor affecting medical research. At present, the research and assessment mechanism of the paper and subject was one-sided. Conclusion: Through creating a favorable academic atmosphere, increasing investment in scientific research, cultivating excellent young backbone of scientific research, establishing scientific research assistant recruitment system, transforming scientific research evaluation model to enhance the level of hospital scientific research and promote the progress of medical research.