To discuss medical staff’s salary level and its satisfaction degree of public hospitals in Nanjing to provide scientific basis for salary system reform, a total of 965 staffs from public hospitals in Nanjing were investigated by questionnaire survey. The results showed: the annual income of medical staff was RMB 88 600±44 800 per person with position difference and hospital grade difference; The expected annual average income of employees was about 2 times of the current average annual salary, and 2 or 3 times of the average social wage; The overall salary level satisfaction score was 3.06±0.89, which was at general level; The satisfaction of the three dimensions was ranked in order of score: internal fairness, individual fairness and external equity. Through multivariate linear regression analysis, the overall satisfaction had significantly difference among age, marital status, technical class, positions and hospital grade(P<0.05). So the salary system reform in public hospitals should strive to improve the salary level, reasonably widen the income gap between different positions, and pay attention to the non?economic compensation, in order to effectively promote the health and sustainable development of public hospitals.