To collect 25 clinical subject benefit data from a large?scale comprehensive public hospital in Nanjing, and to select five dimensions of work quantity, work efficiency, treatment quality, social benefit and scientific research ability, and to evaluate comprehensively the subject benefit using the rank sum ratio method. The subject benefit was divided into high, medium and low level. The analysis of variance (F=45.126, P<0.001) showed that the difference of 3 levels was statistically significant. There were 10 high subject benefits, 11 moderate , and 4 low. This result was basically consistent with the actual situation. Based on the actual situation of the subject development, the disciplinary development proposals were suggested which would comprehensively improve the efficiency of disciplines, and improve the comprehensive strength of hospitals.