The article investigated the explicit and implicit discrimination of college students against hepatitis B virus carriers， to provide empirical evidence for the current prevention and control of hepatitis B. A convenient sampling method was adopted to conduct a questionnaire survey on explicit attitudes among 210 college students in a university in Fuzhou， and 30 college students were randomly selected to measure implicit attitudes with implicit association test. The results showed that 38.4% of the people had hepatitis B discrimination in explicit attitude， which was reflected in four dimensions: marking （39.2%）， stereotyping （47.9%）， differentiation （40.1%） and discrimination（26.4%）， while the D value of implicit attitude test was 0.03， indicating that there has also hepatitis B discrimination attitude. It can be seen that the discrimination of college students against hepatitis B virus carriers has not completely disappeared. Their most obvious stereotype is that they have bad hygiene habits， which leads to their refusal to get close in intimate relationship and living space. However， there is a general consensus among college students on the protection of the right of hepatitis B virus carriers to work and study.