The authors collected information on the level of professional public health institutions， personnel composition and organization type， and analyzed the outflow and changes of personnel in different regions， levels and types of professional public health institutions. The outflow rate of professional public health institutions in Jiangsu province in 2020 was higher than that in 2016. The outflow rates of professional public health institutions at provincial and municipal levels were lower than those at district and county levels. In terms of the types of institutions， the overall personnel outflow rate from high to low was maternal and child health centers， specialized disease prevention and control centers， blood collection and supply institutions， disease prevention and control centers and emergency centers. In terms of economic development levels in 2020， the outflow rate of professional public health institutions in Jiangsu province from high to low was the central ， north and south region. The outflow rate of professional public health institutions in Jiangsu Province showed an increasing trend. The outflow rates at district and county levels were generally higher than those at the provincial and municipal levels. The outflow rate of the centers for disease control and prevention was stable. The personnel outflow in specialized disease prevention and control centers， and maternal and child health centers remained relatively serious. A talent stabilization mechanism for professional public health institutions should be established.