Objective：To explore the effect and mechanism of TIR/BB⁃loop mimetic AS⁃1 on renal ischemia/reperfusion injury in mice. Methods：Male C57BL/6 mice were randomly and equally divided into four groups：sham operation group(Sham group)，renal ischemia/reperfusion injury group(RIR group)，AS ⁃ 1+ renal ischemia/reperfusion injury group(AS ⁃ 1+RIR group)，vehilce + renal ischemia/reperfusion injury group(vehilce + RIR group). The renal ischemia/reperfusion injury model was constructed by clipping bilateral renal veins for 45 min and reperfusion for 24 h. The AS⁃1+RIR group and vehilce+RIR group were intraperitoneally injected with 50 mg/kg AS ⁃ 1 and vehicle 30 minutes before operation. After 24 h reperfusion，renal functional parameters，serum mediator concentrations，markers of apoptosis indexes and expression of phosphorylated NF⁃κB p65 were determined. Results：Serum creatinine (Scr)、blood ureanitrogen(BUN)、TNF⁃α、IL⁃6、Cleaved caspase3、Bax and p⁃NF⁃κB p65 were significantly increased after renal IR compared with the sham group(P＜0.01). The expression of Bcl⁃2 and the ratio of Bcl⁃2/Bax were significantly decreased(P ＜ 0.01). After treatment with AS⁃1，the levels of Scr、BUN、TNF⁃α、IL⁃6、cleaved caspase 3、Bax and P⁃p65 were significantly decreased(P ＜ 0.01)，the expression of Bcl ⁃2 was increased(P ＜ 0.01)，and the ratio of Bcl ⁃2/Bax was also increased. Conclusion：TIR/BB loop analogue AS ⁃ 1 has protective effect on renal ischemia ⁃ reperfusion injury in mice. The mechanism may be related to its anti ⁃ inflammatory and anti apoptotic effects by inhibiting the activation of NF ⁃ κ B signaling pathway.