Objective：This study invented a coaxial dual-channel micro-injection technique to prepare the poly(lactic-co-glycolic acid)(PLGA)microspheres for the first time，which can be used for guiding bone regeneration. Methods：A dual-channel microsyringe was used to prepare PLGA microspheres encapsulated bone morphogenetic protein 2(BMP-2). An optical microscope，a fluorescence microscopy，a scanning electron microscopy(SEM)were used to observe morphology and structure of the microspheres. The changes of morphology and mass loss of the microspheres soaked in PBS solution were recorded for degradation. Cytocompatibility of the microspheres was detected by CCK -8 and cell Calcein/PI staining kit. The effect of PLGA microspheres loading with BMP - 2 on the chemotaxis of MC3T3-E1 cell in 24 hours was detected by Transwell cell migration assay. Results：The microspheres in this study showed regular mono cavity. The mean diameter of the microspheres was 167.58 μm. Two different mean thickness of microsphere shell [(23.82±7.10)μm and(35.31±5.55)μm]can be acquired. The microspheres degraded gradually in 2 months in PBS solution. The results of CCK -8 and ceu Calcein/PI staining test showed cells remained activity with PLGA microspheres. Transwell cell migration assay indicated PLGA microspheres loading with BMP-2 had chemotactic effect on cells in 24 hours. Conclusion：The microspheres in this study with releasing rate of BMP-2 controlled by the thickness of microspheres shell and its surface adhered by cells easily，have apotential of guiding bone regeneration.