Objective：This study aims to evaluate the effect of Parkin mediated mitophagy on globular adiponectin(gAPN) ameliorated H9C2 cardiomyocytes injury induced by intermittent hypoxia(IH)and the potential mechanisms. Methods：The cell IH model was established. H9C2 cell apoptosis was detected by flow cytometry. The mitophagy was detected by fluorescence colocalization of LC3 and mitochondrial red marker. The protein expression levels of Bax，Bcl-2 and Parkin were detected by Western blot. The mitochondrial membrane potential(MMP) was detected by JC-1 kit. Results：After IH exposure，the apoptosis of H9C2 cardiomyocytes，protein expression levels of Bax and Parkin，mitophagy were increased，MMP and Bcl-2 protein expression were decreased. After gAPN treatment，the apoptosis of H9C2 cardiomyocytes，Bax protein expression level were decreased，the MMP， protein expression levels of Bcl-2 and Parkin，and mitophagy were increased. Inhibiting Parkin gene expression decreased the mitophagy，and increased the mitochondrial injury and H9C2 cell apoptosis. Conclusion：gAPN ameliorated the IH induced H9C2 cardiomyocytes injury through upregulating Parkin mediated mitophagy.