新型冠状病毒特异性抗体微流控法定量检测能力评价及应用
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E?mail:zhuliguo2002@163.com

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R446.62

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江苏省社会发展项目(BE2021739)


Evaluation and application of a novel microfluidic method to detect COVID ⁃19 antibodies quantitatively
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    摘要:

    目的:建立并评价新型冠状病毒即严重急性呼吸综合征冠状病毒2型(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)特异性抗体定量检测方法,为新型冠状病毒肺炎(corona virus disease-2019,COVID-19)疫情防控提供血清学研判支持。方法:采集2020年1—2月SARS-CoV-2感染者及其密切接触者的血清和咽拭子样本,分别用RT-qPCR法检测SARS- CoV-2核酸,微流控法和胶体金法检测SARS-CoV-2特异性抗体,并比较分析检测结果的一致性。结果:微流控法、胶体金法检测SARS-CoV-2特异性IgG抗体的结果均与RT-qPCR方法一致。发病早中期(<14 d)与后期(≥14 d)微流控法IgG抗体阳性率差异有统计学意义。结论:微流控法是一种新型可定量的SARS-CoV-2抗体检测法,检测结果准确且耗时短。

    Abstract:

    Objective:This study aims to establish and evaluate antibody quantitative detection methods of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),in order to provide serological data base for corona virus disease-2019(COVID-19)detection and prevention. Methods:The serum and throat swab samples of SARS-CoV-2 infected persons and their close contacts from January to February 2020 were collected,and the SARS-CoV-2 nucleic acid was detected by RT-qPCR,the SARS-CoV-2 specific antibody was detected by microfluidic method and colloidal gold method,respectively,and the consistency of the results was compared and analyzed. Results:The results of detection of SARS - CoV - 2 specific IgG antibody by microfluidic method and colloidal gold method were consistent with those by RT-qPCR,but there were significant differences in IgG positive rates between the early-middle and the late stages by the microfluidic method. Conclusion:As a novel quantitative way to detect SARS-CoV-2 antibodies,the microfluidic method is brief and accurate.

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秦圆方,彭杰夫,田华,蒋屹,陈思羽,朱立国.新型冠状病毒特异性抗体微流控法定量检测能力评价及应用[J].南京医科大学学报(自然科学版),2022,42(11):1621-1626

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  • 在线发布日期: 2022-11-24
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