An efficient double ovulation protocol for simultaneous induction of multicellular embryos and fertilized eggs in adult mice
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摘要:
目的：探讨二次超排方案对提高成年小鼠超排效果和胚胎发育潜能的可行性。方法：首先比较常规双排卵方案与自然排卵、随机超排实验方案所获总卵数的差异，然后在初步研究外源孕马血清促性腺激素(pregnant mare serum gonadotro- pin，PMSG)对自然受孕一次胚胎发育影响的基础上，进一步对常规双排卵实验方案进行优化。结果：优化后的双排卵实验组单只鼠平均总卵数高达61.7枚，与自然排卵组、随机超排组和常规双排卵组差异显著，提升了成年小鼠的超排卵效果。结论： 优化后的超数双排卵方案为同步大量获得小鼠多细胞胚胎及受精卵提供了一种新的、高效的技术方法。
Abstract:
Objective：To investigate the feasibility of secondary superovulation scheme in improving the superovulation effect and embryonic development potential of adult mice. Methods：Firstly，the difference of total egg number among the conventional double ovulation scheme，the natural ovulation and random superovulation experimental schemes was compared，and then the conventional double ovulation experimental scheme was further optimized on the basis of the preliminary study of the effect of exogenous pregnant mare serum gonadotropin(PMSG)on the development of naturally conceived embryos. Results：The average total number of eggs in the optimized double ovulation experimental group was 61.7，which was significantly different from the natural ovulation group，the random superovulation group and the conventional double ovulation group，and improved the superovulation effect of adult mice. Conclusion： The optimized double superovulation scheme provides a new and efficient technical method for obtaining a large number of mouse multi -cell embryos and fertilized eggs.
