Objective：The current study aims to explore the effect of bergapten(BP)on the osteogenic differentiation of human dental pulp stem cells(hDPSCs). Methods：Specific antigens of hDPSCs were identified by flow cytometry analysis. The cytotoxicity and biosafety of BP was detected by CCK - 8. The hDPSCs were randomly divided into the control and BP groups. Different concentrations of BP(7.5 μmol/L，15.0 μmol/L and 30.0 μmol/L)were added to complete medium in the BP groups，while an equal volume of DMSO was added to the complete medium in the control group. The morphology of hDPSCs in the BP and control groups was observed by fluorescent stain. The BCIP/NBT staining and alkaline phosphatase(ALP)quantitative detection were used to detect the effect of BP at the early stage of osteogenic differentiation of hDPSCs. The alizarin red S(ARS)staining and semi-quantitative analysis was used to assess the degree of extracellular matrix mineralization at the late stage of osteogenic differentiation. Expressions of osteogenic-related genes such as Runt-related transcription factor 2(RUNX2)，osteocalcin(OCN)，osteopontin(OPN)，and ALP were detected by quantitative real -time PCR(qRT - PCR). Expressions of osteogenic - related proteins were detected using Western blot. Results：BP of 7.5 μmol/L，15.0 μmol/L and 30.0 μmol/L showed better biosafety(P ＜ 0.01). The ALP activities in the BP groups were higher than that in the control group，and the 15.0 μmol/L BP was the best(P ＜ 0.01). The degrees of extracellular matrix mineralization of the BP groups were higher than that of the control group，and the number of calcium nodules in the 15.0 μmol/L BP treatment group was more and deeper. The expressions of osteogenic-related genes and proteins were significantly increased in the BP groups and the 15.0 μmol/L BP treatment was the best(P ＜ 0.01). Conclusion：BP shows good biosafety and can promote the osteogenesis differentiation of hDPSCs with better effect at the 15.0 μmol/L concentration in vitro