Objective：This study aims to investigate the relevant factors of bone substitute materials retention rate after implant surgery with guided bone regeneration in the aesthetic zone. Methods：From January 2019 to December 2021，the cone beam CT data of 50 patients who received implant surgery in the aesthetic zone with guided bone regeneration in the implant department of the Affiliated Stomatological Hospital of Nanjing Medical University were collected before the operation，immediately after the operation and six months after the operation. Three-dimensional model reconstruction of the patient’s maxilla is performed in mimics19.0，along with registering three reconstructed models and calculating the retention rate of bone substitute materials，and its correlation with other clinical data was analyzed. Results：The retention rate of bone substitute materials in six months after the operation is statistically correlated with the use of concentrated growth factor，alveolar ridge splitting technique and thrombin time(P ＜ 0.05). There is a linear negative correlation between thrombin time and the retention rate of bone substitute materials(P ＜ 0.01). Conclusion：Both concentrated growth factor and alveolar ridge splitting technique are associated with the increase of bone substitute materials’retention rate in six months after the implant surgery，while long thrombin time is correlated with low bone substitute materials’retention rate.