Objective：To investigate the effects of exogenous interleukin(IL)- 25 in gut damage in mice caused by Schistosoma japonicum(S.japonicum)infection. Methods：Twenty - four female C57BL/6J mice were randomly divided into four groups，control group(n=5)，control+IL-25(n=5)，S.japonicum infection group(Inf，n=7)，S.japonicum infection +IL-25 group(Inf+IL-25，n=7). Mice in the Inf group and Inf+IL-25 group were infected with forty S. japonicum cercariaes. IL-25 was intraperitoneally injected from the 4th week after infection(0.5 μg/mice every 2 days for 3 weeks). Six weeks after infection，mice were sacrificed. Hematoxylin eosin(HE) staining was performed in the liver and colon tissue to observed the pathological changes. Alcian blue-periodic and acid-Schiff(AB-PAS) staining was performed to observe the number of goblet cells in the colon. The levels of inflammation-related cytokines including IL-10， IL-4，IL-13，tumor necrosis factor α(TNF-α)，interferon γ(IFN-γ)，IL-1β in the colon were detected by enzyme linked immunosorbent assay(ELISA)and real - time polymerase chain reaction(real - time PCR). Results：HE staining results showed that the area of individual granulomas in the colon in the Inf+ IL -25 group was significantly lower than that of mice in the Inf group，but the area of granulomas in the liver in the Inf group was not significantly different from that in the Inf+IL-25 group. The results of AB-PAS staining showed that IL-25 could significantly promote the number of colonic goblet cells in mice after infection with S.japonicum，the ELISA and the real-time PCR results showed that cytokines in the colon after S.japonicum infection were obviously altered，and IL-25 injection promoted the expression of type 2 cytokines and decreased the expression of type 1 cytokines. Conclusion：IL-25 can alleviate the gut damage induced by S.japonicum infection by promoting the differentiation of goblet cells.