Expression of lncRNA RP11⁃490M8.1 in breast cancer and its roles in the proliferation of breast cancer cells
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摘要:
目的：确定lncRNA RP11-490M8.1在乳腺癌中的表达情况及其在乳腺癌细胞增殖中的作用。方法：基于癌症基因图谱(The Cancer Genome Atlas，TCGA)数据库和Kaplan-Meier plotter数据库分析RP11-490M8.1在乳腺癌中的表达情况及与患者预后的关系；qRT-PCR检测RP11-490M8.1在乳腺癌和癌旁组织中的表达；CCK8和克隆形成实验检测过表达和敲低RP11- 490M8.1对乳腺癌细胞增殖的影响。结果：与癌旁组织相比，RP11-490M8.1在乳腺癌组织中表达明显下调，且RP11-490M8.1 低表达和较差的总生存期(overall survival，OS)密切相关(P=0.034)；RP11-490M8.1表达水平与乳腺癌患者的临床分期呈负相关，而与雌激素受体、孕激素受体、人表皮生长因子受体-2的表达以及年龄均无相关性；过表达RP11-490M8.1显著抑制乳腺癌细胞的增殖，敲低RP11-490M8.1表达则促进其增殖。结论：RP11-490M8.1在乳腺癌中可能发挥抑癌基因的作用，是潜在的乳腺癌预后分子和治疗靶点。
Abstract:
Objective：To investigate the expression of lncRNA RP11-490M8.1 in patients with breast cancer and its roles in the proliferation of breast cancer cells. Methods：The Cancer Genome Atlas(TCGA)and the Kaplan -Meier plotter database were used to define the expression level and prognostic value of RP11-490M8.1 in breast cancer，respectively. qRT-PCR was performed to detect the expression level of RP11-490M8.1 in paired breast cancer tissues and adjacent normal tissues. CCK8 and clone formation assays were carried out to determine the effects of the overexpression or knockdown of RP11-490M8.1 on the proliferation of breast cancer cells. Results：Compared with adjacent normal tissues，RP11-490M8.1 was significantly downregulated in breast cancer tissues. Moreover， lower expression of RP11-490M8.1 was associated with the advanced clinical stage and worse overall survival(OS)(P=0.034). However，there was no correlation between RP11-490M8.1 level and the expression of estrogen receptor，progesterone receptor，human epidermal growth factor receptor-2 and age. Overexpression of RP11-490M8.1 markedly suppressed the proliferation of breast cancer cells，while knockdown of RP11-490M8.1 promoted its proliferation. Conclusion：RP11-490M8.1 acts as a tumor suppressor in breast cancer，which may serve as a potential prognostic molecule and therapeutic target for breast cancer
