Objective：The current study aims to explore the expression of hsa_circ_0001479 in gastric cancer(GC)tissues and its effect on biological behavior of GC cells. Methods：The method for detecting the expression of hsa_circ_0001479 with real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was established and evaluated. Expression level of hsa_circ_0001479 in 76 pairs of GC and para-cancerous tissues was measured by RT-qPCR and relationship between the expression level and clinicopathological factors was analyzed. Small interfering RNA(siRNA)and overexpression vector of hsa_circ_0001479 were constructed and transfected into GC cell lines. Cell proliferation was detected by CCK-8 assay and clone formation assay. Cell cycle distribution was detected by flow cytometry. Cell migration was detected by wound healing assay and Transwell assay. Cell invasion was detected by transwell assay. Results：Using RT-qPCR to detect the expression of hsa_circ_0001479 possessed good precision， accuracy and linear range. Hsa_circ_0001479 was upregulated in GC tissues and the expression level was related to tumor size，depth of invasion，lymph node metastasis，TNM stage and CA19-9. Silencing of hsa_circ_0001479 inhibited the proliferation，invasion and migration of GC cells while overexpression of hsa_circ_0001479 showed the opposite results. Conclusion：The carrent study found that hsa_circ_0001479 was upregulated in GC tissues and could promote GC cell proliferation，invasion and migration.