The self-renewal，proliferation and differentiation of spermatogonial stem cells are critical for maintaining spermatogenesis. However，the molecular mechanism mediating this process remains largely unclear. Spermatogonial stem cells include different cell subtypes which play different roles in spermatogenesis. Single-cell RNA sequencing technology provides an effective method to reveal different subtypes of spermatogonia and their changes in transcriptome levels and biological functions，which could provide new perspectives for investigaing further molecular mechanisms of spermatogonial stem cells.