Objective：To investigate the neural circuit of depressive symptoms derived from glucocorticoid（GC）receptor in dorsal raphe nucleus. Methods：Firstly，a stress mouse model was established using a single stimulus coufinement method，and the mice were divided into four groups：control group，stresssd group，mifepristone-treated group and vehicle group，then the mice were injected with GC receptor antagonists to test behavioral changes by tail suspension test and elevated plus maze test. Immunofluorescence was utilized to detect the activation of GC receptors and microgial. Results：①Compared with the control group，the immunofluorescence result showed that the number of GC receptors in dorsal raphe nucleus was increased after stress stimulate. ②After intraperitoneal injection of the GC receptor antagonists（mifepristone，RU486），the amount of GC receptors and the activated microglial cells were decreased in the dorsal raphe nucleus of the mice，and the depression-like behaviors were also attenuated. Conclusion：The above results indicated that mifepristone regulates microglial cell activation in dorsal raphe nucleus to eventually reduce depression-like behavior.