Objective：To investigate the effects of bisphenol F（BPF）on the tryptophan（TRP）and tyrosine（TYR）- derived neurotransmitter metabolism in the liver，elucidating the metabolic characteristics of the neurotransmitter disturbance elicited by BPF. Methods：The SPF C57BL/6J mice were administrated with different concentrations of BPF[40，400，4 000 μg/（kg·d）]by gavage for 30 consecutive days，and the ultra -high -performance liquid chromatography -tandem mass spectrometry（UHPLC -MS/MS）assay was used to examine the characteristics and levels of TRP and TYR - derived neurotransmitters in the mouse livers after BPF exposure. Results：The levels of TRP and TYR in mouse livers were significantly decreased after BPF exposure ，and 5-hydroxytryptamine （5 - HT）was significantly decreased，while kynurine（KYN），another key metabolite of the TRP metabolic pathway，significantly increased. For the TYR metabolism，TYR and its metabolite dopamine（DA）obviously decreased；meanwhile，other excitatory neurotransmitters，such as glutamate（GLU），γ - aminobutyric acid（GABA），aspartic acid（ASP）and glycine（GLY），dramatically decreased. Conclusion：BPF exposure can decrease the levels of TRP，TYR and several other excitatory neurotransmitters，and promote the metabolic transformation of 5-HT into KYN in the TRP pathway. Therefore，the current study on the metabolism of hepatic neurotransmitters provides novel evidence for the liver and neuropsychiatric diseases caused by continuous exposure to BPF.