Objective：To investigate the relationship between TLR2/NF-κB signaling pathway and the apoptosis and autophagy dysfunction of RSC96 cells induced by 1-methyl-4-phenyl-pyridinium（MPP⁺ ）. Methods：RSC96 cells were divided into the PBS group， MPP ⁺ group，and MPP ⁺ + CU-CPT22 group. Cell survival rate was detected using CCK-8 after treatment with different MPP ⁺ concentrations（0.1，0.3，0.5，0.7，0.9 mmol/L）. Cell apoptosis was detected by TUNEL staining. RT-qPCR was performed to detect the TLR2 mRNA level. Western blot was performed to detect the expression levels of apoptosis related indicators Bcl-2/Bax，cleaved caspase-3/caspase-3，autophagy-related indicators LC3II/LC3I and P62，as well as TLR2 and p-NF-κB/NF-κB. Results：Compared with the PBS group，the cell viability of the MPP ⁺ group decreased in a concentration-dependent manner，the number of TUNEL-staining positive cells increased，the ratio of Bcl-2/Bax decreased while cleaved caspase-3/caspase-3 ratio increased，as well as had a decrease in the ratio of LC3Ⅱ/LC3Ⅰ and an increase in P62 and p -NF -κB/NF -κB ratio elvel. RT -qPCR and Western blot results showed that MPP ⁺ upregulated the expression of TLR2. In addition，compared with the MPP ⁺ group，the MPP ⁺ + CU-CPT22 group showed a decrease in the number of TUNEL-staining positive cells，an increase in Bcl-2/Bax level，and a decrease in cleaved caspase-3/ caspase-3 ratio. Meanwhile，LC3 Ⅱ/LC3 Ⅰ ratio was increased，and the P62 expression level was decreased. Conclusion：MPP ⁺ stimulation induced apoptosis and the imbalance of autophagy in RSC96 cells，and the mechanism may be related to the activation of the TLR2/NF-κB signaling pathway.