Objective：To detect the residual levels of environmental pollutants in the bodies of biomedical laboratory researchers in Jiangsu Province and provide a basis for the establishment of laboratory biosafety regulations. Methods：A total of 100 biomedical laboratory researchers and 100 non-researchers were selected as subjects from universities in four regions of Jiangsu Province：Nanjing， Huai’an，Yancheng，and Suzhou. A questionnaire survey was conducted to understand the current exposure to environmental pollutants among different groups. Morning urine samples were collected from the participants，with 95 samples from researchers and 94 samples from non -researchers. The urinary levels of di（2 - ethylhexyl）phthalate（DEHP），bisphenol A（BPA），and benzo[a]pyrene were measured using β-glucuronidase hydrolysis and high-performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）. Results：The levels of DEHP，BPA，and benzo[a]pyrene in the urine of biomedical laboratory researchers in Jiangsu Province [（1 359.0±251.0）μg/g，（284.4±134.0）μg/g，（109.0±43.2）ng/g]were significantly higher than those in the control group’s urine [（489.7±103.1）μg/g，（177.3±86.4）μg/g，（80.2±31.5）ng/g，P ＜ 0.001]. Benzo[a]pyrene levels were higher in the urine of laboratory researchers in Nanjing and Suzhou[（132.4±37.1）ng/g，（139.3±32.8）ng/g]，while DEHP showed no significant differences among laboratory researchers in different regions. However，the levels of BPA in laboratory researchers from Yancheng were the highest [（386.1 ± 202.1）μg/g]. Conclusion：Biomedical laboratory researchers in various regions of Jiangsu Province have higher accumulations of environmental pollutants in their bodies compared to non -laboratory personnel. This could be associated with their daily laboratory work，emphasizing the need for laboratory researchers to pay more attention to protecting themselves from environmental pollutants.