负载仙茅苷的3D打印复合支架促进血管化和成骨效应的研究
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R681

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常州市科技计划资助(CE20215020);南京医科大学常州医学中心科研项目(CMCC202217)


Research on 3D printed composite scaffolds loaded with curculigoside for promoting vascularization and osteogenesis
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    摘要:

    目的:研究负载仙茅苷的三维复合支架的理化特性,并评估其对人脐带静脉内皮细胞(human umbilical vein endo- thelial cell,HUVEC)和小鼠骨髓间充质干细胞(bone marrow mesenchymal stem cell,BMSC)促进血管化和骨诱导方面的潜在作用。方法:采用乳液/溶剂蒸发技术制备负载仙茅苷(curculigoside,CUR)的聚己内酯微球(CUR-PM),借助3D生物打印技术成功构建了由羟基磷灰石(hydroxyapatite,HA)、明胶(gelatin,GEL)和海藻酸钠(sodium alginate,SA)组成的三维复合(hydroxyapa- tite gelatin sodium alginate,HGS)支架的基础上,制备了负载仙茅苷的聚己内酯微球(hydroxyapatite gelatin sodium alginate curcu- ligoside,HGSC)支架。通过扫描电镜、红外光谱、流变学、力学性能、药物释放和降解性等实验对支架进行表征;通过CCK-8、 EdU荧光染色实验以验证支架的生物安全性;通过HUVEC细胞管形成实验、碱性磷酸酶(alkaline phosphatase,ALP)染色,评估 HGSC用于促进细胞血管化和成骨调控的潜能。结果:HGSC内部具有大小均匀的网格状结构,与HGS相比,具有适当的力学性能和降解性。CCK-8和EdU荧光染色结果显示,HGSC支架具有良好的生物相容性。HUVEC细胞管形成实验和BMSC的 ALP染色结果表明,HGSC支架表现出促进血管化和骨形成的潜能。结论:HGSC支架具备良好的生物相容性,并且对BMSC的骨诱导潜能和HUVEC的血管化具有明显的促进作用,为骨缺损修复提供了具有前景的治疗策略。

    Abstract:

    Objective:This study aimed to investigate the physicochemical properties of a three-dimensional(3D)composite scaffold loaded with curculigoside(CUR)and assess its potential impact on promoting angiogenesis and osteogenic induction in human umbilical vein endothelial cells(HUVEC)and mouse bone marrow mesenchymal stem cells(BMSC). Methods:Polycaprolactone microspheres loaded with Curculigoside(CUR-PM)were prepared using an emulsion/solvent evaporation technique. We successfully engineered a 3D composite scaffold comprising hydroxyapatite(HA),gelatin(GEL),and sodium alginate(SA)with the assistance of 3D bioprinting technology,denoted as HGS. Furthermore,we established a polycaprolactone-based microsphere scaffold,referred to as HGSC,for the purpose of CUR loading. The scaffold was characterized by scanning electron microscopy,infrared spectroscopy, rheology,mechanical properties,drug release,and degradation experiments. The biocompatibility of the scaffold was verified using CCK-8 and EdU fluorescence staining experiments. The potential of the HGSC scaffold to promote cell vascularization and bone regulation was assessed through HUVEC tube formation experiments and alkaline phosphatase(ALP)staining. Results:The HGSC exhibited a uniform grid-like structure with appropriate mechanical properties and degradation compared to HGS. The CCK-8 and EdU fluorescence staining demonstrated excellent biocompatibility of the HGSC scaffold. The outcomes of HUVEC tube formation experiments and BMSC ALP staining provided evidence that the HGSC scaffold exhibited the potential in promoting angiogenesis and osteogenesis. Conclusion:The HGSC scaffold has shown excellent biocompatibility and has a clear promoting effect on the osteogenic potential of BMSC and angiogenesis of HUVEC. This study offers a promising therapeutic strategy for bone defect repair.

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袁秀琛,范世杰,段伟豪,谭亚东,吴璟斌,周瑞凯,翁益平.负载仙茅苷的3D打印复合支架促进血管化和成骨效应的研究[J].南京医科大学学报(自然科学版),2024,(2):162-169

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  • 收稿日期:2023-09-17
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  • 在线发布日期: 2024-02-05
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