Objective：To explore the expression and significance of arachidonate 12-lipoxygenase（ALOX12）in cancer tissues of colon cancer patients. Methods：Tumor and peritumoral tissues of 257 colon cancer patients were retrospectively collected，and the expression of ALOX12 was detected by immunohistochemistry（IHC）to compare the differences in expression within the tumor and peritumoral tissues，and the relationship between ALOX12 expression and clinicopathological features of colon cancer was analyzed. The 5-year overall survival（OS）of the patients was analyzed using Kaplan-Meier curves，and the factors affecting the 5-year OS of the patients were investigated by univariate and multivariate Cox regression analyses. Results：ALOX12 was more positively expressed in tumors compared to peritumoral tissues（63.8% vs. 33.1%），and the difference was statistically significant（P < 0.001）. The expression of ALOX12 was not significantly correlated with the patient’s age，gender，and tumor site（P > 0.05），but was strongly correlated with TNM staging and lymph node metastasis（P < 0.05）. Five-year OS of patients with high expression of ALOX12 was markedly lower than those patients with low expression of ALOX12（P < 0.001）. Single variable and multivariate Cox analyses indicated that age，TNM staging，lymph node metastasis，and ALOX12 expression were independent factors influencing the 5-year OS rate（P < 0.05）. Conclusion：High expression of ALOX12 is associated with TNM staging and lymph node metastasis of colon cancer，which can serve as a biomarker to predict the patient’s condition and prognosis.
[3] GALLUZZI L，VITALE I，AARONSON S A，et al.Molecular mechanisms of cell death：recommendations of the nomenclature committee on cell death 2018[J].Cell Death Differ，2018，25（3）：486-541
[4] CHEN X，YU C，KANG R，et al.Iron metabolism in ferroptosis[J].Front Cell Dev Biol，2020，8：590226
[5] ZHENG Z，LI Y，JIN G，et al.The biological role of arachidonic acid 12⁃lipoxygenase（ALOX12）in various human diseases[J].Biomed Pharmacother，2020，129：110354
[6] SHAO Y，JIA H，HUANG L，et al.An original ferroptosis⁃related gene signature effectively predicts the prognosis and clinical status for colorectal cancer patients[J].Front Oncol，2021，11：711776
[7] CHU B，KON N，CHEN D，et al.ALOX12 is required for p53 ⁃ mediated tumour suppression through a distinct ferroptosis pathway[J].Nat Cell Biol，2019，21（5）：579-591
[8] YANG X H，ZHUANG M K，XIE W H，et al.12⁃Lipoxy⁃genase promotes epithelial ⁃ mesenchymal transition via the Wnt/β ⁃ catenin signaling pathway in gastric cancer cells[J].Onco Targets Ther，2019，12：5551-5561
[9] FEDCHENKO N，REIFENRATH J.Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue ⁃ a review[J].Diagn Pathol，2014，9（1）：221
[10] CHUANG J P，TSAI H L，CHEN P J，et al.Comprehensive review of biomarkers for the treatment of locally advanced colon cancer[J].Cells，2022，11（23）：20
[11] BENSON A B，VENOOK A P，CEDERQUIST L，et al.Colon cancer，version 1.2017，NCCN clinical practice guidelines in oncology[J].J Natl Compr Canc Netw，2017，15（3）：370-398
[12] WANG Y H，ZHANG Z Y，SUN W C，et al.Ferroptosis in colorectal cancer：potential mechanisms and effective therapeutic targets[J].Biomed Pharmacother，2022，153：14
[13] CHENG Z，CHEN Y H，HUANG H C.Identification and validation of a novel prognostic signature based on ferroptosis⁃related genes in ovarian cancer[J].Vaccines，2023，11（2）：20
[14] YANG J H，WEI X H，HU F，et al.Development and validation of a novel 3⁃gene prognostic model for pancreatic adenocarcinoma based on ferroptosis ⁃ related genes[J].Cancer Cell Int，2022，22（1）：18
[15] LUO H，MA C.A novel ferroptosis⁃associated gene signature to predict prognosis in patients with uveal melanoma [J].Diagnostics，2021，11（2）：25
[16] SONG J H，LIU Y H，GUAN X，et al.A novel ferroptosis⁃related biomarker signature to predict overall survival of esophageal squamous cell carcinoma[J].Front Mol Biosci，2021，8：13
[17] WENG S Y，LIU Z Q，XU H，et al.ALOX12：a novel insight in bevacizumab response，immunotherapy effect，and prognosis of colorectal cancer[J].Front Immunol，2022，13：15
[18] SHEN H，GENG Z，NIE X，et al.Erianin induces ferroptosis of renal cancer stem cells via promoting ALOX12/P53 mRNA N6 ⁃ methyladenosine modification[J].J Cancer，2023，14（3）：367-378
[19] SARSOUR E H，SON J M，KALEN A L，et al.Arachido⁃nate 12⁃lipoxygenase and 12⁃hydroxyeicosatetraenoic acid contribute to stromal aging⁃induced progression of pancreatic cancer[J].J Biol Chem，2020，295（20）：6946-6957
[20] CHEN J，TONG W，LIAO M，et al.Inhibition of arachido⁃nate lipoxygenase 12 targets lung cancer through inhibiting EMT and suppressing RhoA and NF⁃κB activity[J].Biochem Biophys Res Commun，2020，524（4）：803-809
