Obesity is caused by a long-term excess of energy intake over expenditure which leads to the storage of excess energy as fat in adipose tissue or other tissues. The activation of adipose tissue dependent adaptive thermogenesis could increase energy expenditure and improve glucose and lipid metabolism，which is promising target for obesity. Both brown adipocytes and beige adipocytes participate in thermogenesis. Thermogenic adipocytes in adults are more close to beige adipocytes which have the capability to switch between an energy storage and energy dissipation phenotype，and could be activated by cold or adrenegic stimuli. Mitochondrial protein uncoupling protein 1 in thermogenic adipocytes could dissipate energy as heat. Recent studies have found that mitochondrial dynamics，quality control，metabolites and itself as the signaling organelles play vital roles in regulating beige biogenesis and function. This review will discuss the characteristics of mitochondrial and how mitochondrial regulate beige adipose tissue.