Objective：To investigate the correlation between the expression of integrin binding sialoprotein（IBSP）in glioblastoma （GBM）and the clinical prognosis of patients，and its regulatory effect on tumor cell proliferation，invasion and chemotherapy resistance. Methods：A public database was used to analyze the expression of IBSP in GBM and its relationship with the prognosis of patients. After IBSP was knockdown or overexpressed in GBM cells，functional assays were used to assess cell proliferation，invasion， and chemoresistance. Results：Database analysis showed that the expression level of IBSP in GBM was significantly increased，and the high level of IBSP indicated a poor prognosis. Knockdown of IBSP inhibited GBM proliferation，invasion and chemoresistance，and overexpression of IBSP promoted GBM proliferation，invasion and chemoresistance. Overexpression of IBSP promoted the activation of NF -κB pathway and the expression of downstream genes of NF -κB，which in turn promoted the malignant phenotype of GBM cells. Conclusion：IBSP promotes GBM cell proliferation，invasion and chemoresistance by activating the NF-κB signaling pathway， suggesting that IBSP can be used as a potential therapeutic target for GBM.