化妆品中3种常见抗生素的一种快速检测方法
作者:
作者单位:

南京医科大学公共卫生学院卫生检验与检疫学系,江苏 南京 211166

中图分类号:

R115

基金项目:

江苏省大学生创新创业训练计划(202310312001Z)


A rapid detection method of three common antibiotics in cosmetics
Author:
Affiliation:

Department of Health Inspection and Quarantine,School of Public Health,Nanjing Medical University,Nanjing 211166 ,China

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    摘要:

    目的:建立一种针对化妆品中3种常见抗生素(氯霉素、氧氟沙星和头孢克肟)的微生物定性检测方法。方法:配制含抗生素的化妆品标准溶液以模拟实际的化妆品样品的背景基质,选用改良Letheen肉汤培养基中和样品中的防腐剂,以样品、培养基和金黄色葡萄球菌菌液(菌液浓度约为1×1011 cfu/mL)体积比1∶9∶2为检测体系,37 ℃培养45 min和90 min后,通过紫外可见分光光度计检测细菌回收率,与标准回收率进行比较后进行定性判断。结果:本方法对氯霉素、氧氟沙星和头孢克肟的最低检测量分别为5 μg/g、1 μg/g和1 μg/g。结论:本方法操作简单,成本低廉,可用于化妆品中3种常见抗生素的快速检测。

    Abstract:

    Objective:To establish a microbiological qualitative detection method for three antibiotics(chloramphenicol,ofloxacin, and cefixime)in cosmetics. Method:A cosmetic standard solution containing antibiotics was prepared to simulate the background matrix of actual cosmetic samples. Modified Letheen broth(MLEB)was used to neutralize preservatives in the samples. The volume ratio of the sample,MLEB and bacterial solution(concentration was approximately 1×1011 cfu/mL)was 1∶9∶2. After cultured at 37 ℃ for 45 and 90 minutes,the bacterial recovery rate was analyzed and compared with the standard recovery rate in order to make qualitative judgments. Results:The detection limit of this method for chloramphenicol,ofloxacin and cefixime were 5 μg/g,1 μg/g,and 1 μg/g,respectively. Conclusion:This method is convenient,cheap,accurate,and suitble for the rapid detection of 3 common antibiotics in cosmetics.

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成治纬,卫昕怡,王婉莹,卞心如,仲国维,张晓玲.化妆品中3种常见抗生素的一种快速检测方法[J].南京医科大学学报(自然科学版),2025,(2):233-239

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  • 收稿日期:2024-06-21
  • 在线发布日期: 2025-02-19
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