With the development of China"s medical and health undertakings, it puts forward higher requirements on the indicators of In-vitro diagnostic technique such as sensitivity,limit of detection and analysis speed. However, traditional in-vitro diagnostic techniques are increasingly unable to meet this certain requirement. In the meanwhile, the combination of nanomaterials and in-vitro diagnostic techniques has increasingly attracted researchers’ attention. In this article, we have reviewed the latest advances in the application of nanomaterials in in-vitro diagnostic analysis.