Abstract: Objective To investigate the glucose metabolism characteristics of CD4+CD25+CD127low Treg cells (Regulatory T cells, Treg) and CD4+CD25-CD127+ Teff cells (Effector T cells, Teff) in growth environment of ovarian cancer cells. Methods FACS (Fluorescence-activated cell sorting, FACS) separated CD4+ Treg cells and CD4+ Teff cells, then established a coculture system of human T cells with ovarian cancer cell SKOV3. The expression levels of glucose metabolism related genes and proteins were determined by RT-PCR (real-time fluorescence quantitative PCR, RT-PCR) and Western Blot. The levels of glucose uptake and glycolysis were detected by colorimetry. Results Compared with the CD4+ Treg cells in single culture without SKOV3 and CD4+ Teff cells , the expression levels of glucose metabolism related genes and proteins in CD4+ Treg cells cocultured with SKOV3 were elevated (P＜0.05) and the levels of glucose uptake and glycolysis were increased (59.778±2.579 pmol vs. 44.927±1.862 pmol，P＜0.05；15.308 ± 2.052 mM vs. 7.975 ± 0.878 mM，P＜0.05). Conclusions The level of glucose metabolism in CD4+ Treg cells is higher than CD4+ Teff cells in ovarian cancer cell growth environment, ovarian cancer cells could promote the expression levels of glucose metabolism related genes and proteins in CD4+ Treg cells.