Objective:This study aims to investigate the value of bile acid profile in diagnosis of intrahepatic cholestasis of pregnancy and asymptomatic hypercholanemia of pregnancy. Methods:100 cases of normal pregnancy, 60 cases of ICP and 30 cases of AHP were selected. The content of bile acid in each group was analyzed by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). In addition, the orthogonal projections to latent structures (OPLS-DA) model was developed to explore the differences of bile acids between the ICP and AHP groups.The diagnostic effiency of the differential bile acids and the combined indicators was analyzed by receiver operating characteristic（ROC）curves.Results :Except for chenodeoxycholic acid (CDCA), the contents of 15 kinds of bile acids among the three groups were statistically significant (P < 0.05).According to the OPLS-DA model, we found that the different bile acids in ICP group and AHP group were taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), glycocholic acid (GCA), glycohenodeoxycholic acid(GCDCA), tauroursodeoxycholic acid (TUDCA).ROC results showed that TCA had the highest diagnostic efficiency with area under curve (AUC) of 0.808. The combination of TCA, TCDCA, gcdca and TUDCA was more effective in the diagnosis of ICP. The AUC was 0.967, the sensitivity was 90.6%, and the specificity was 98.0%.Conclusion:There are differences in bile acids between ICP and AHP. The differential bile acids found by OPLS-DA model can be used as an important method for differential diagnosis between ICP and AHP.