Objective To evaluate the consistency and correlation of TRAb measured by automated electrochemiluminescence immunoassay and radioreceptor assay, and whether two methods can be used interchangeably in clinical work through quantitative analysis. Methods 122 serum samples from patients for TRAb measurement were assayed measuring with automated electrochemiluminescence immunoassay and radioreceptor assay simultaneously between September to November 2018. Kappa, Passing-bablok and Bland Altman analyses were performed and the proportional differences between two methods were assayed. Results The Kappa value of TRAb measured between two methods was 0.84, and the Spearman's correlation coefficient was 0.91, which was highly correlated. The Passing-bablok regression formula for TRAb mesurement was automated electrochemiluminescence immunoassay level = -0.9 +1.8× radioreceptor assay level, indicating that on average the TRAb levels measured with automated electrochemiluminescence immunoassay were 80% - 0.9 IU/L higher than radioreceptor assay levels. Bland Altman suggested that the absolute difference between the two methods increased with increasing TRAb levels. Conclusion The TRAb levels measured with automated electrochemiluminescence immunoassay and radioreceptor assay are highly correlated and consistent, but there is a proportion difference between them in quantitative comparison. In the process of hyperthyroidism, the two methods are inappropriate to evaluate the remission interchangeably.