Objective: This study aims to explore the expression of hsa_circ_0001479 in gastric cancer (GC) tissues and its effect on biological behavior of GC cells. Methods: The method for detecting the expression of hsa_circ_0001479 using real-time fluorescence quantitative PCR (RT-qPCR) was established and evaluated via experiments；Expression level of hsa_circ_0001479 in 76 pairs of GC and para-cancerous tissues was measured by RT-qPCR and relationship between expression level and clinicopathological factors was analyzed；Hsa_circ_0001479 interference siRNA and overexpression vector were constructed and transfected into GC cell lines；Cell proliferation was detected by CCK-8 assay and clone formation assay; cell cycle distribution was detected by flow cytometry, cell migration was detected by wound healing assay and Transwell assay; cell invasion was detected by Transwell assay. Results: Using RT-qPCR to detect the expression of hsa_circ_0001479 possessed good precision, accuracy and linear range；Hsa_circ_0001479 was upregulated in GC tissues and the expression level was related to tumor size, depth of invasion, lymph node metastasis, TNM stage and CA19-9；Silencing of hsa_circ_ 0001479 inhibited the proliferation, invasion and migration of GC cells while overexpression of hsa_circ_0001479 showed the opposite results. Conclusion: This study found that hsa_circ_0001479 was upregulated in GC tissues and could promote cell proliferation, invasion and migration..