环状RNA hsa_circ_0001479在胃癌中的表达及功能研究
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南通大学附属医院检验科

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R446.1

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中国博士后科学基金资助的项目


Expression and function of hsa_circ_0001479 in gastric cancer
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China Postdoctoral Science Foundation funded project

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    摘要:

    目的:探讨hsa_circ_0001479在胃癌组织中的表达情况及其对胃癌细胞生物学行为的影响。方法:建立实时荧光定量PCR(RT-qPCR)检测hsa_circ_0001479表达量的方法并进行方法学性能评价;通过RT-qPCR检测76对胃癌组织和配对癌旁组织中hsa_circ_0001479的表达水平并分析其表达量与临床病理参数的关系;构建hsa_circ_0001479干扰片段和过表达载体,转染至胃癌细胞株,通过CCK-8实验、克隆形成实验检测细胞增殖能力,流式细胞术检测细胞周期分布,划痕实验检测细胞横向迁移能力,Transwell实验检测细胞侵袭和迁移能力。结果:RT-qPCR检测hsa_circ_0001479表达量的方法具有良好的精密度、正确度和线性; Hsa_circ_0001479在胃癌组织中表达增高且表达水平与肿瘤大小、浸润深度、淋巴结转移、TNM分期和CA19-9水平有关;细胞功能实验显示,在胃癌细胞株中干扰hsa_circ_0001479的表达可抑制胃癌细胞的增殖、侵袭和迁移,过表达hsa_circ_0001479则呈现相反的结果。结论:本研究发现胃癌组织中高表达的hsa_circ_0001479具有促进胃癌细胞增殖、侵袭和迁移的功能。

    Abstract:

    Objective: This study aims to explore the expression of hsa_circ_0001479 in gastric cancer (GC) tissues and its effect on biological behavior of GC cells. Methods: The method for detecting the expression of hsa_circ_0001479 using real-time fluorescence quantitative PCR (RT-qPCR) was established and evaluated via experiments;Expression level of hsa_circ_0001479 in 76 pairs of GC and para-cancerous tissues was measured by RT-qPCR and relationship between expression level and clinicopathological factors was analyzed;Hsa_circ_0001479 interference siRNA and overexpression vector were constructed and transfected into GC cell lines;Cell proliferation was detected by CCK-8 assay and clone formation assay; cell cycle distribution was detected by flow cytometry, cell migration was detected by wound healing assay and Transwell assay; cell invasion was detected by Transwell assay. Results: Using RT-qPCR to detect the expression of hsa_circ_0001479 possessed good precision, accuracy and linear range;Hsa_circ_0001479 was upregulated in GC tissues and the expression level was related to tumor size, depth of invasion, lymph node metastasis, TNM stage and CA19-9;Silencing of hsa_circ_ 0001479 inhibited the proliferation, invasion and migration of GC cells while overexpression of hsa_circ_0001479 showed the opposite results. Conclusion: This study found that hsa_circ_0001479 was upregulated in GC tissues and could promote cell proliferation, invasion and migration..

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  • 收稿日期:2021-06-18
  • 最后修改日期:2021-09-04
  • 录用日期:2023-05-11
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