Abstract: Objective: The study aims to investigate the role of Zfp212 on female fertility by generating Zfp212 knockout mice model.Methods: CRISPR/Cas9 system was used to generate Zfp212 knockout mice.Zfp212 expression pattern, subcellular location and Zfp212 knockout efficiency were detected by quantitative real-time PCR (RT-qPCR),immunofluorescence staining (IF) and Western blot(WB).Ovary histological analysis and HE staining,in vitro fertilization (IVF),in vitro embryo culture and fertility test were performed to explore the effects of Zfp212 knockout on female mice fertility.Results: Zfp212 was expressed dominantly in oocytes and early embryos,and it showed a maternal expression pattern.The results of WB and sanger sequencing showed Zfp212 knockout mice model was constructed successfully.Compared with control groups,Zfp212 knockout female mice had no significant differences in follicle development,oocyte maturation,fertilization,preimplantation embryo development and litter size.Conclusion: These results indicated that Zfp212 gene is not necessary for the establishment of female fertility in mice.