Objective: To investigate the feasibility and therapeutic effect of minimally invasive surgery in treatment of gynecomastia. Methods: 37 patients diagnosed with gynecomastia (a total of 61 breasts) treated with minimally invasive surgery in Breast Surgery Department, the First Affiliated Hospital with Nanjing Medical University from June 2018 to September 2020 were included in this retrospective study. Results: Minimally invasive surgery was successfully performed to all the 37 patients under general anesthesia. A mean operative duration of 78.0 minutes per side was required (40~125 min). The intraoperative blood loss was 15 to 200ml with the mean of 54.1ml. Three postoperative bleeding (4.92%), 2 reversible paresthesia of the nipple (3.28%), and 2 skin redundancy (3.28%) were encountered. None of the patients experienced severe pain, infection, nipple necrosis, a saucer-like deformity, limited breast mobility and recurrence. With a mean follow-up of 9.9 months (6~12 months), patient satisfaction is 100%. Conclusion: The minimally invasive surgery can be used for the treatment of gynecomastia, which may need further research and it is worthy to be promoted.