Objective: Compare the effects of the immediate and delayed autotransfusion of residual cardiopulmonary bypass pump blood on the hemostasis time of the aortic dissection that received deep hypothermic circulatory arrest. Methods: 128 cases, who underwent the aortic dissection with hypothermia circulatory arrest in our hospital during 2018 and 2020, were retrospectively summarized. They were divided into immediate group and delayed group according to the time of residual blood autotransfusion from cardiopulmonary bypass. The hemoglobin level and coagulation function before and after the surgery, intraoperative fluid management and hemostasis time were compared between the two groups. Results: A total of 128 cases were enrolled, 60 in the immediate group and 68 in the delayed group. The level of hemoglobin in both groups was significantly reduced (P<0.01), but the decline is less in the delayed group (P<0.05). And there was no significant change in coagulation function in the two groups before and after the surgery. In the delayed group, the use of blood products was decreased (P<0.05), the bleeding was decreased (P<0.01), and the hemostasis time, operation time and anesthesia time was shortened (P<0.01). Conclusion: The delayed autotransfusion of residual cardiopulmonary bypass pump blood reduced intraoperative bleeding and shortened the hemostasis time in the aortic dissection with hypothermic circulatory arrest.