Objective: This study invented a coaxial dual-channel micro-injection technique to prepare a hollow poly(lactide-co-glycolide) (PLGA) microsphere for the first time, which can be used for guiding bone regeneration. Methods: A dual-channel microsyringe was used to prepare PLGA microspheres encapsulated BMP-2. An optical microscope, a fluorescence microscopy, a scanning electron microscopy were used to observe morphology and structure of microspheres. The change of morphology and mass loss of microspheres soaked in PBS solution was recorded for degradation. CCK-8 and Dead/Live kit was adopted to test cytocompatibility of microspheres. The effect of PLGA microspheres loading with BMP-2 on the chemotaxis of MC3T3-E1 cell in 24 hours was detected by transwell cell migration assay. Results: The microsphere in this study showed regular mono cavity. The mean diameter of microspheres was 150μm. Two different mean thickness of microsphere shell can be acquired. The microspheres degraded gradually in 2 months in PBS solution. The results of CCK-8 and Dead/Live test showed cells can remain activity with PLGA microspheres and transwell cell migration assay indicated PLGA microspheres loading with BMP-2 had chemotactic effect on cells in 24 hours. Conclusion: The microspheres in this study with its releasing rate of BMP-2 controlled by the thickness of microspheres shell and cells easily adhered to its surface, have a potential of guiding bone regeneration.