To investigate the detection rate and influencing factors of thyroid nodules among healthy teachers in our hospital. Methods: To retrospectively analyze the examination items (including gender, age, occupation, height, weight, etc.), laboratory examination (fasting blood glucose, blood lipids, liver function, uric acid, C13 breath test, etc.) and thyroid ultrasonography were retrospectively analyzed in the health teacher physical examination population of our hospital from October 2018 to June 2021. Logistic multifactor regression was used to analyze the influencing factors of thyroid nodules. Results: 1. Thyroid nodules were found in 2539 of 6096 patients, with a total detection rate of 41.65%, including 38.20% male and 43.03% female. There were 1340 single nodules (21.98%) and 1199 multiple nodules (19.67%). For thyroid nodule classification according to Ti-RADS, grade 3 was the most, accounting for 22.74%. The older the age, the higher the detection rate was, and there were significant differences among different age groups (P<0.05). In addition, thyroid nodules were detected in bilateral lobe (17.27%) > right lobe (13.27%) > left lobe (10.89%) > thyroid isthmus (0.21%). 2. The detection rate of female teachers in middle education group was the highest (49.44%), and the detection rate of different types of teachers in the two age groups of 30 years old and 30-39 years old had statistical difference (P<0.05) 3. Compared with the non-thyroid nodule group, the incidence of hypertension, abnormal fasting glucose, hyperlipidemia, overweight and obesity was higher in the two groups, and the difference was statistically significant (P<0.05). 4. Logistic multifactor regression analysis showed that female was an independent risk factor for thyroid nodules (OR=1.913, P<0.05), and teachers aged 40-49, 50-59 and ≥60 had a higher risk of thyroid nodules than those aged less than 30 (OR=1.402, 2.923, 4.637, All P<0.05), and compared with preschool education group, college teachers had a lower risk of thyroid nodules (OR=0.718, P<0.05). Conclusion: In our hospital, the detection rate of thyroid nodules among teachers who underwent physical examination is high, but the malignancy is not high. Overweight, obesity and "three high" are closely related to thyroid nodules. Female, age and middle school teachers are risk factors for thyroid nodules, so comprehensive attention should be paid to the above indicators to improve health management awareness.