Abstract：Objective To establish and evaluate antibody quantitative detection methods of COVID-19 (Corona Virus Disease 2019), in order to provide serological data base for COVID-19 detection and prevention. Methods Between January and February 2020, to gather serum and pharyngeal swab samples of COVID-19 patients and their close contacts, then to test sera using Microfluidic and colloidal gold methods and nucleic acid using RT-qPCR (reverse transcription-quantitative Real-time PCR) respectively. Finally, the consistency of two sorts of results were analyzed and compared. Results There was no significant difference between the IgG (immunoglobulin G) antibody positive rates of Microfluidic method, colloidal gold method and the positive rates of RT-qPCR, but significant differences in IgG positive rates between the early-middle and the late stages by the Microfluidic method was noted. Conclusion As a novel quantitative way to detect COVID-19 antibodies, the microfluidic method is brief and accurate. Preliminarily verified by this study, this method is applied to quantify COVID-19 antibodies. For more practical applications, it requires more researches to confirm.