Abstract:Abstract Objective: To explore the effect of overexpressing or silencing long intergenic non-protein coding RNA 01518 (LINC01518) on the cell proliferation induced by IL-17 in non-small cell lung cancer (NSCLC). Methods: The IL-17 receptor A (IL-17RA) expression in NSCLC cell lines (PC9, H1299 and H1975) was first examined using Western blot. Then H1299 and PC9 cells were exposed to IL-17 for different time, and the cell proliferation was detected by CCK8 assay. Based on the results of up-regulated lncRNAs detected by lncRNA microarray in IL-17-stimulated H1299 and PC9 cells, we selected and verified several lncRNAs expression level by RT-PCR and Real-time PCR. Additionally, we transfected the constructed plasmids of pcDNA3.1/LINC01518 or shLINC01518 into H1299 cells, and examined the parameters of H1299 cell proliferation by CCK8, EdU and colony formation assays. Results: IL-17RA could express in 3 NSCLC cell lines. The cell proliferation and LINC01518 were obviously up-regulated in H1299 and PC9 cells treated by IL-17. Besides, LINC01518 overexpression could markedly promote the cell proliferation, while LINC01518 gene knockdown followed by IL-17 treatment did not enhance the level of cell proliferation. Conclusion: LINC01518 can promote H1299 cell proliferation induced by IL-17 stimulation.