Objective: To explore the surgical experience of a domestic single-port robotic surgical system in extraperitoneal radical prostatectomy. Methods: From November 2021 to July 2022, 9 patients with prostate cancer were scheduled for radical prostatectomy according to the criteria of the nanorobot. A domestic single-port robotic surgical system was used to complete radical prostatectomy using a transperitoneal approach. Information on incision size, operative time, operating time, and intraoperative blood loss was recorded. Besides, perioperative data, surgical complications and postoperative recovery were also analyzed. Results: The mean age of the 9 patients was 74.11 years (range: 66 to 85 years), mean BMI value of 23.62 (18.3 to 29.4), mean operative time of 168.0 min (125 to 249 min), mean operation time of 119.2 min (85 ~ 194min), mean preparation time of 12.4min (5 ~ 36min), and the mean incision size of 4.22cm (3.5 ~ 5cm). While, no cases had added auxiliary channels. The average postoperative hospital stay was 19 days (range: 12 to 26 days). For urinary control, 3 patients was restored immediately after surgery and 6 patients restored in one month after surgery. All the patients were pathologically confirmed as prostatic adenocarcinoma, among which 2 cases were found with positive margins. No complications such as bleeding, fever, and incision infection was found in patients in the postoperative period. Conclusion: Using the domestic single-port robotic surgical system could safely and effectively complete single-port extraperitoneal radical prostatectomy. In addition, the serpentine arm had the advantages of flexible movement and accurate pointing, which can guarantee the smooth implementation of surgery.