Abstract Backgrounds and Aims: The role of abnormal brain-gut interaction mechanism in functional gastrointestinal disorders (FGIDs)has been recognized widely. As a part of the brain-gut axis , autonomic nervous function (ANS),which is controlled emotion, may affect the anorectal physiology function and disease state of constipation patients. However, the correlations between them remain unclear. The aim of this study was to evaluate the associations between ANS function and constipation symptoms, psychological status and quality of life in function constipation (FC) patients. Methods: We retrospectively analyzed the clinical data of 81 FC patients, who were eligible in our study. They were divided into three groups according to the result of heart rate variability(HRV) test: sympathovagal balance normal group ，balance severely sympathetic group and balance severely vagal group. The results of Self-Rating Anxiety and Depression Scale (SAS/SDS), Constipation Scoring System (CSS), Patient Assessment of Constipation Symptom (PAC-SYM), as well as the Quality of life(PAC-QOL) were compared between groups. Meanwhile, anorectal manometry and electrointestinogram parameters were assessed. We also analyzed the potential association between PAC-QOL and ANS function, mental status, constipation symptoms in all the constipation patients. Result: Anorectal physiology and intestinal electric activity showed no significant difference between the groups. Compare with patients in the normal group, patients in the sympathetic group had higher SAS/SDS scores, higher PAC-SYM score and lower PAC-QOL, especially for the dimension of “physical discomfort” and “worry/anxiety” of PAC-QOL. The vagal group showed higher CSS score than the other two groups. Maybe depressed emotion is prominent. Multiple regression analysis showed that anxiety/depression emotion, ANS function state can significantly affect the quality of life.