This study aims to establish and evaluate epileptic kindled by kainic acid (KA) microinjected into piriform cortex. Methods: Using c-fos immunofluorescence staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining techniques to find the piriform cortex associated with epilepsy in a mouse model of acute epilepsy. Racine score and seizure latency were observed after injection of kainic acid in piriform cortex. Behavioral experiments included testing the learning and memory abilities of mice in a water maze and testing the exploration ability of mice in a new object recognition experiment. The results of seizures and behavioral experiments were compared with the epileptic model of stereotactic injection of kainic acid into the hippocampus. The electrical activities of new model mouse neurons were measured by electrophysiological techniques. Results: The results of c-fos immunofluorescence and TUNEL staining showed that in addition to the hippocampus, neurons in the piriform cortex and hippocampus were also significantly activated and apoptosis occurred in the acute epilepsy mouse model; After injecting KA into the piriform cortex, the epilepsy was successfully ignited. Compared with the epilepsy model injected KA into the hippocampus, there was no significant difference in the epileptic seizure score and the seizure latency was shorter; The behavioral results showed that the epileptic model mice injected KA into the piriform cortex had a reduced spatial memory ability and a reduced ability to explore new objects. These results showed no significant difference compared to the epileptic model injected KA into the hippocampus. Patch clamp recording test showed an increase of firing frequency and the resting membrane potential, and a decrease of amplitude of neuronal discharge. Conclusion: Microinjection of KA into the piriform cortex has successfully induced epilepsy, which provides a new animal model for studying the pathogenesis of epilepsy.